Part:BBa_K1441003:Design

PGAPz alpha B + Linker

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 764
  Illegal XbaI site found at 787
  Illegal SpeI site found at 802
  Illegal PstI site found at 823
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 764
  Illegal SpeI site found at 802
  Illegal PstI site found at 823
  Illegal NotI site found at 775
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 764
  Illegal BglII site found at 1
  Illegal BamHI site found at 1241
  Illegal XhoI site found at 736
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 764
  Illegal XbaI site found at 787
  Illegal SpeI site found at 802
  Illegal PstI site found at 823
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 764
  Illegal XbaI site found at 787
  Illegal SpeI site found at 802
  Illegal PstI site found at 823
  Illegal NgoMIV site found at 1998
  Illegal NgoMIV site found at 2059
  Illegal AgeI site found at 960
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 1224
  Illegal BsaI.rc site found at 1423
  Illegal BsaI.rc site found at 2420
  Illegal SapI site found at 244
  

Design Notes

We replaced old multiple cloning cite with new modified multiple cloning site, which has new cutting sites according to iGEM standard. The original MCS has ECORI, KpnI, NotI and XbaI restriction enzyme binding sites compared to the new MCS we designed in which there are ECORI, NotI, XbaI, SpeI and PstI restriction enzyme cutting sites.

Source

None